Determination of cytochrome P-448 activity in biological tissues
نویسندگان
چکیده
منابع مشابه
Studies on the synthesis of cytochrome P-450 and cytochrome P-448 in rat liver.
The synthesis of cytochrome P-450 (phenobarbital inducible) and cytochrome P-448 (3-methylcholanthrene inducible) have been studied in rat liver in vivo and in the wheat germ cell-free system using anti-cytochrome P-450 and anti-cytochrome P-448 antibodies. The major mature forms synthesized in vivo correspond to a molecular weight of 47,000 for cytochrome P-450 and 53,000 for cytochrome P-448....
متن کاملHigh resolution gas chromatographic analysis of cytochrome P-448 inducing polychlorinated biphenyl congeners in hazardous waste.
Polychlorinated biphenyls (PCBs), which have been commercially manufactured since 1929, are complex mixtures of many congeners and several homologues. They have been used in a variety of industrial and non-industrial applications which has allowed large quantities to be released into the workplace and environment1-6. Based on a 198 1 estimate, as many as 17 million pounds of PCBs were entering ...
متن کاملPolychlorinated biphenyls: a new type of inducer of cytochrome P-448 in the liver.
The CO-difference spectrum of microsomes from rats treated with the polychlorinated biphenyls mixture, Aroclor 1254, has an absorption maximum at 448 nm. With ethylisocyananide as the ligand for reduced microsomes, Aroclor 1254 treatment causes a shift in the 455-nm peak to 453 nm and increases the ratio of absorbance of 455 nm to that at 430 nm from 0.53, obtained with untreated rats, to 1.24....
متن کاملQuantification of cytochrome P-450 gene expression in human tissues.
Humans can metabolize an extremely wide range of potentially harmful foreign hydrophobic compounds including many drugs, environmental pollutants and chemical carcinogens. This ability is mainly due to the action of cytochromes 1’-450. These proteins constitute a large superfamily that has been classified into several families, the largest of which (P45011) has been further divided into seven s...
متن کاملStudies on the substrate-binding sites of liver microsomal cytochrome P-448.
The interaction of substrates of the microsomal mixed-function oxidases with cytochromes P-450 and P-448 was investigated by using liver microsomes from rats pretreated with phenobarbital or 3-methylcholanthrene, and with purified forms of the cytochromes isolated from rabbit liver. The two forms of the cytochrome have different substrate specificities; cytochrome P-450 has one type 1 substrate...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1984
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2210081